Aim of present study was to explore the interaction potential of Glycyrrhiza glabra with Solanum xanthocarpum on antimicrobial activity. To derive possible magnitude of interaction at various combinations, Checkerboard microtitre test was performed and Fractional Inhibitory Concentration Combination Index values (FICI) were used to analyze and compare the different combination effects between the herbs tested. The results showed that antibacterial effects of 80% ethanolic extract of both herbs against S abony, E. coli, B. Pumulus, P.aeruginosa, M. luteus were concentration dependant. Both the extracts showed significant (p< 0.01) antibacterial activity as compared to control. In present study two-fold serial dilution technique was used to determine the minimal inhibitory concentration (MIC) of Solanum xanthocarpum extract (SXE) and Glycyrrhiza glabra extract (LE) against the selected bacterial strains. The results revealed that the SXE have antibacterial effects against S abony, E. coli, B. Pumulus, P.aeruginosa, M. luteus at concentration levels of 750, 1000, 500, 750 and 500 µg/ml, respectively. While LE have antibacterial effects against S abony, E. coli, B. Pumulus, P.aeruginosa, M. luteus at concentration levels of 250, 500, 125, 500 and 250µg/ml, respectively. Results of checkerboard assays suggested that MIC of LE combined with SXE was remarkably decreased. Moreover, Bacterial susceptibility to SXE and LE was extremely improved by combination. FIC indices indicated the synergism between SXE and LE against S abony, E. coli, B. Pumulus, P.aeruginosa, M. luteus, with an FIC index of 0.583, 0.75, 0.562, 0.833 and 0.5 respectively. In conclusion, we suggest the combination of SXE and LE for the treatment of S abony, E. coli, B. Pumulus, P.aeruginosa, M. luteus may reduce the efficacious dose of SXE and LE alone and thus minimize the side-effects. Further clinical experiments are necessary to assess the potential for therapeutic application.
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